Analiza antimikrobne prevlake sa površine medicinskog tekstila metodom tankoslojne kromatografije

master's thesis

• Author: Petra Kirin
• Mentor: Iva Rezić (mentor)
• Committee member: Maja Somogyi Škoc (predsjednik povjerenstva)
• Committee member: Iva Rezić (član povjerenstva)
• Committee member: Mario Cetina (član povjerenstva)
• Granter: University of Zagreb; Faculty of Textile Technology; (Department of Applied Chemistry); Zagreb
• Defense date and country: 2019-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Textile Technology

Abstract

U radu je prikazano ispitivanje sadržaja mikrokapsula alginata punjenih nanočesticama srebra koje pokazuju izuzetno snažna antimikrobna i antibakterijska svojstva, a koje su pogodne za nanašanje na tekstilne materijale i funkcionaliziranje površine. Nanočestice srebra imaju anti-bakterijsko djelovanje na širok spektar Gram-pozitivnih i Gram-negativnih bakterija, uključujući spojeve rezistentne na antibiotike. Imaju veliku primjenu zbog svoje kemijske stabilnosti, kata-litičke aktivnosti, i visoke vodljivosti te izuzetnu antimikrobnu aktivnost zbog visokog omjera površine i volumena, što im osigurava bolji kontakt s mikroorganizmima. Metode inkapsulacije koriste se u tekstilnoj, farmaceutskoj i kozmetičkoj industriji kako bi dostavile biološki aktivne supstance na željena mjesta. Dostupnost novih aktivnih nanočestica otvorila je mogućnosti razvoja novih matrica i primjena koje se mogu koristiti u razvoju raznih proizvoda, a inkapsuli-ranje je relativno nova tehnologija koja omogućuje čuvanje, stabilnost i sporo otpuštanje akti-vnih tvari. Materijale za oblikovanje mikrokapsula uglavnom čine ugljikohidrati (škrob, celuloza, gume), proteini (proteini mlijeka - kazeini i proteini sirutke, gluten, želatina) i lipidi (masne ki-seline, alkoholi, gliceridi, voskovi, fosfolipidi) ili njihova kombinacija. Mikrokapsule postaju sve prisutnije u funkcionalizaciji tekstilnih polimera. Tehnike inkapsuliranja bioaktivnih sastojaka obuhvaćaju koacervaciju, mikrokapsuliranje raspršivanjem u struji toplog zraka, mikrokapsuli-ranje fluidiziranih čestica, mikrokapsuliranje ekstruzijom i superkritičnim fluidima, emulgira-njem i kokristalizacijom. U ovom radu su priređene mikrokapsule ispitivane metodom tanko-slojne kromatografije kako bi se odredio sadržaj srebra na uzorcima. Kromatografska metoda pokazala se kao vrlo brza, učinkovita i ekonomski isplativija metoda od mnogih instrumentalnih metoda te se preporuča za korištenje u mnogim ispitnim laboratorijima.

Abstract (english)

This work presents thin layer chromatographic determination of the content of alginate micro-capsules filled with silver nanoparticles that exhibit extremely strong antimicrobial and antiba-cterial properties, which are suitable for application on textile materials and their surface fun-ctionalization. Silver nanoparticles have anti-bacterial effects on a wide range of Gram-positive and Gram-negative bacteria, including antibiotic-resistant strains. They are widely used beca-use of their chemical stability, catalytic activity, and high conductivity, as well as their excepti-onal antimicrobial activity due to their high surface-to-volume ratio, which provides them with better contact with microorganisms. Encapsulation methods are used in the textile, pharmace-utical and cosmetic industries to deliver biologically active substances to desired places. The availability of new active nanoparticles has opened up opportunities for the development of new matrices and applications that can be used in the development of various products, and encapsulation is a relatively new technology that enables the storage, stability and slow rele-ase of active substances. Materials for forming microcapsules are mainly carbohydrates (starch, cellulose, gums), proteins (milk proteins - caseins and whey proteins, gluten, gelatin) and lipids (fatty acid-line, alcohols, glycerides, waxes, phospholipids) or a combination thereof. Microcapsules are becoming increasingly present in the functionalization of textile polymers. Techniques for encapsulating bioactive constituents include co-conservation, micro-encapsu-lation by hot-air flow, micro-encapsulation of fluidized particles, micro-encapsulation by extru-sion and supercritical fluids, emulsification and cocrystallization. In this work, microcapsules were investigated by thin layer chromatography to determine the silver content of the samples. The chromatographic method has proven to be a very fast, efficient and cost-effective method advantageous over many other instrumental methods and can be threfore recommended for usage in many test laboratories.

• Keywords: mikrokapsulacija; antimikrobna zaštita; srebro; kozmetotekstilije; tankoslojna kromatografija
• Keywords (english): microencapsulation; antimicrobial protection; silver; cosmetic textile; thin layer chromatography
• Language: croatian
• URN:NBN: urn:nbn:hr:201:221150
• Study programme: Title: Textile Technology and Engineering; specializations in: Clothing Engineering, Textile Design and Management, Industrial Textile Design, Industrial Clothing Design, Textile Chemistry, Materials and Ecology; Course: Textile Chemistry, Materials and Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka tekstilne tehnologije i inženjerstva (magistar/magistra inženjer/inženjerka tekstilne tehnologije i inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-10-31 12:28:08