Antimikrobna učinkovitost prirodnog bojila iz lišća biljke Azadirachta indica nanesenog na plazmom aktiviranu površinu celuloznih pletiva

master's thesis

• Author: Tea Vujičić
• Mentor: Sanja Ercegović Ražić (mentor)
• Committee member: Ana Sutlović (predsjednik povjerenstva)
• Committee member: Sanja Ercegović Ražić (član povjerenstva)
• Committee member: Martinia Ira Glogar (član povjerenstva)
• Granter: University of Zagreb; Faculty of Textile Technology; (Department of Materials, Fibres and Textile Testing); Zagreb
• Defense date and country: 2020-10-30, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Textile Technology; Textile Chemistry

Abstract

U današnjem svijetu je vrlo potrebno antimikrobno sredstvo koje je ekološko, ekonomski prihvatljivo i pogodno za ljudsko zdravlje. U ovom radu se istraţivala uĉinkovitost lista od biljke Nim (l. Azadirachta indica), kao antimikrobno sredstvo za obradu tekstilnih materijala (pletiva) iz pamuĉnih i liocelnih vlakana. Za potrebe istraţivanja upotrebljen je suhi ekstrakt lista biljke Nim koji je prireĊen u obliku alkoholne otopine definirane koncentracije i u definiranom omjeru se koristio za obradu pletiva. Uzorci su prethodno obradi, predobraĊeni moĉilom (KAl(SO4)2 i kisikovom i argonovom plazmom u definiranim uvjetima, nakon ĉega je provedena obrada iscrpljenjem kupelji prirodnog obojenog ekstrakta optimirane koncentracije, sa i bez primjene moĉila razliĉite koncentracije.
Nakon obrada provedena je analiza makrostrukture površine uzoraka primjenom USB mikroskopa Dino-lite, analiza antimikrobne uĉinkovitosti primjenom kvalitativnog mikrobiološkog testa, te kolorimetrijska analiza otopina i obojenja uzoraka, apsorpcijskim i remisijskim spektrofotometrom. Na uzorcima pletiva iz liocelnih vlakana dobiveno je obojenje ţutog tona, a kod uzoraka pletiva iz pamuka obojenje zelenkastog tona. Uzorci predobraĊeni plazmom (bez obrade moĉilom) daju najbolje rezultate antimikrobne uĉinkovitosti spram ciljanih bakterija Staphylococcus aureus i Klebsiella pneumoniae. Analizom otopina primjenom apsorpcijskog spektrofotometra potvrĊen spoj nimbolid koji je odgovoran za antibakterijska svojstva lišća nima, te klorofil a - odgovoran za obojenje ekstrakta. Na temelju dobivenih rezultata moţe se zakljuĉiti da je ekstrakt iz lista biljke Nim sigurno, ekološko i ekonomski pogodno prirodno sredstvo s dobrim antimikrobnim djelovanjem, koje se moţe koristiti kao prirodno bojilo za celulozne materijale.

Abstract (english)

In today's world, an antimicrobial agent that is environmentally, economically acceptable and suitable for human health is very much needed. In this paper, the effectiveness of the list of Nim plants (l. Azadirachta indica) as an antimicrobial agent for the processing of textile materials (knit) from cotton and lyocell fibers was investigated. For the purposes of the research, a dry extract of the leaves of the Nim plant was used, which was prepared in the form of alcoholic solutions of a defined concentration and used in a defined ratio for the processing of knit. The samples were pre-treated with mordant (KAl(SO4)2 and oxygen and argon plasma under defined conditions, after which the treatment was carried out by exhaustion from the bath with natural colored extract of optimum concentration, with and without mordant, different concentrations.
After processing, the macrostructure analysis of the sample surfaces was performed on the example of a USB microscope Dino-lite, the analysis of antimicrobial efficacies using a qualitative microbiological test, and colorimetric analysis of solutions and staining of samples, with absorption and remission spectrophotometer. Yellow tones are obtained on samples of knits from lyocell fibers, and greenish tones were obtained on samples of cotton knits. Plasma pretreated samples (without mordant) give the best results of antimicrobial efficacy against target bacterias Staphylococcus aureus and Klebsiella pneumoniae. Analysis of the solution using an absorption spectrophotometer confirmed the compound nimbolid, which is responsible for the antibacterial properties of neem leaves, and chlorophyll a - responsible for coloring the extract. Based on the obtained results, it can be concluded that Nim is a safe, ecologically and economically suitable natural agent with good antimicrobial activity, which can be used as a natural dye for cellulosic materials.

• Keywords: Nim (l. Azadirachta indica); antimikrobna uĉinkovitost; predobrade plazmom
• Keywords (english): Neem (l. Azadirachta indica); antimicrobial efficacy; plasma pretreatment
• Language: croatian
• URN:NBN: urn:nbn:hr:201:589836
• Study programme: Title: Textile Technology and Engineering; specializations in: Clothing Engineering, Textile Design and Management, Industrial Textile Design, Industrial Clothing Design, Textile Chemistry, Materials and Ecology, Textile Chemistry, Materials and Ecology; Course: Clothing Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka tekstilne tehnologije i inženjerstva (magistar/magistra inženjer/inženjerka tekstilne tehnologije i inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2021-02-05 12:56:41